Simultaneous Targeted and Unknown Screening using the 3200 QTRAP LC/MS/MS System and Cliquid Software Screening Workflows for Targeted and Unknown Analytes, using Cliquid® 3.2 Software with MS/MS Library Searching Confirmation
نویسنده
چکیده
In this technical note, a novel methodology is described which combines targeted and unknown screening in a single LCMS/MS analysis, using a QTRAP® hybrid tandem mass spectrometer / linear ion trap. In addition to providing screening for target and unknown analytes, this method also generates fullscan MS/MS spectra for the detected analytes, to be used for confirmation via searching against an MS/MS spectral library. The combination of targeted and unknown screening in a single LC-MS/MS analysis saves time, requires less sample volume, and affords the analyst with the opportunity to perform retrospective data mining to extract valuable information.
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